We will investigate the biological sprout control of an invasive alien species, Rosa rugosa. Roses will be cut and an inoculum medium including hyphae of a decay fungus Chondrostereum purpureum will be spread on freshly cut stump surfaces. Our aim is to find out whether repeated treatments will be more efficient than the treatment that will be done only once. Results of this study could potetially be utilized in biological sprout control of woody invasive plant species in the future.