Genome editing via the CRISPR/Cas9 method offers a focused and efficient method without the introduction of additional mutations. The method relies on double-strand break repair, whereby the Cas9 nuclease is used to induce a break in the DNA of the target organism. We propose to establish the infrastructure at Luke to carry out CRISPR/Cas9 –mediated genome editing in plants, using barley as our initial target organism, but then expanding to potato and hops.
Development of CRISPR/Cas9 components.
Preparation of genome editing experiments.
Phenotyping of genome-edited plants.